Chinese Journal of Tissue Engineering Research ›› 2015, Vol. 19 ›› Issue (8): 1255-1260.doi: 10.3969/j.issn.2095-4344.2015.08.020

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Osteoprotegerin expression in the peri-implant sulcus fluid after mechanical stimulations at different angles  

Fang Ming1, Wang Zhi-ying2, Yu Jin2, Li Min2, Jin Ding2   

  1. 1Bohai University, Jinzhou 121000, Liaoning Province, China; 2Center of Dental Implant, the 2nd Affiliated Hospital, Liaoning Medical University, Jinzhou 121001, Liaoning Province, China
  • Revised:2014-12-15 Online:2015-02-19 Published:2015-02-19
  • Contact: Fang Ming, Bohai University, Jinzhou 121000, Liaoning Province, China
  • About author:Fang Ming, Master, Associate professor, Bohai University, Jinzhou 121000, Liaoning Province, China
  • Supported by:

    General Program of Liaoning Provincial Education Department, No. L2011199

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Abstract:

BACKGROUND: Because of the limitation of local anatomical structure, 15° to 30° angled abutments are commonly used for implant restoration. Under the condition of oversized angle mechanical force, the ability of the dental implant to resist invasion of patho-microorganism is poorer than natural teeth, which is decided by the

structural characteristics of peri-implant tissue interface.
OBJECTIVE: To study concentration changes of osteoprotegerin and receptor activator of nuclear factor-kappa B ligand (RANKL) from peri-implant sulcus fluid of cranine mandibular implants under 100 N forces at 0° and 30° angle.
METHODS: Six dogs (18 dental implants) were randomly divided into three groups: group A, 100 N 0° group, group B, 100 N 30° group, and group C, non-mechanical stimulation group (control group). The second premolars of the bilateral mandible were extracted, and two dental implants per side were implanted. Cobalt-chromium PFM full crown was used for tooth restoration 3 months later. After 1 week of full crown restoration, under general anesthesia of 5% odium pentobarbital, 100 N was given vertically (0° angle) and at 30° angle, and once worked for 5 seconds on implants, totally 10 minutes. After 24 hours, 72 hours and 1 week of mechanical force, samples of groups A, B and C were collected from the peri-implant sulcus fluid, and ELISA method was used to detect RANKL and osteoprotegerin levels.
RESULTS AND CONCLUSION: After application of 100 N mechanical force vertically, the RANKL level in the peri-implant sulcus fluid increased obviously at 24 hours, increased continuously at 72 hours, and decreased slightly at  1 week, and there was statistically significance compared to the non-mechanical stimulation group (P < 0.05). After dental implant was given 100 N at 30° angle, RANKL level in the peri-implant sulcus fluid raised significantly compared to 100 N 0° angle group, and there was remarkable significance compared to the non-mechanical stimulation group (P < 0.01). The osteoprotegerin level in the peri-implant sulcus fluid decreased after 24 hours of mechanical stimulation at  0° angle, continued to decline at 72 hours, and returned to 24-hour level after 1 week mechanical force application, and there was no statistical difference compared to non-mechanical stimulation group (P > 0.05). The osteoprotegerin level in the peri-implant sulcus fluid declined gradually over time after 100 N mechanical stimulation at 30° angle, and there was statistically significance compared to the non-mechanical stimulation group (P < 0.05). The results indicate that there are increased bone destructive activities from the peri-implant sulcus fluid under 100 N mechanical stimulation at 30° angle, and it is recommended that the occlusion force works along the direction of dental implant axis clinically.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程


全文链接：

Key words: Dental implants, Osteoprotegerin, RANK Ligand

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